Journal: Allergologia et immunopathologia

Article Title: Alpinia officinarum Hance extract relieved sepsis-induced myocardial ferroptosis and inflammation by inhibiting lncRNA MIAT/TRAF6/NF-κB axis.

doi: 10.15586/aei.v52i5.1035

Figure Lengend Snippet: Figure 2 Alpinia officinarum Hance extract inhibited sepsis-induced myocardial ferroptosis. Groups were divided into the Sham, LPS, LPS+25 mg/kg AOE, LPS+50 mg/kg AOE, and LPS+100 mg/kg AOE groups. (A) The Fe2+ level was evaluated through the corresponding commercial kit. (B) The levels of MDA and SOD were examined through ELISA. (C) The protein expressions of ACSL4 and GPX4 were tested through western blot.

Article Snippet: Detection of Fe2+ The Fe2+ assay kit (E-BC-K304-S, Elabsciences, China) was used for measuring Fe2+ level as per manufacturer’s protocols.

Techniques: Enzyme-linked Immunosorbent Assay, Western Blot

Journal: Allergologia et immunopathologia

Article Title: Alpinia officinarum Hance extract relieved sepsis-induced myocardial ferroptosis and inflammation by inhibiting lncRNA MIAT/TRAF6/NF-κB axis.

doi: 10.15586/aei.v52i5.1035

Figure Lengend Snippet: Figure 5 Overexpression of MIAT reduced the cardioprotective effect of Alpinia officinarum Hance extract. Groups were separated into the Sham, LPS, LPS+100 mg/kg AOE, and LPS+100 mg/kg+Ad-MIAT groups. (A) The protein expressions of TRAF6, p-P65, and P65 were verified through western blot. (B) The level of CK-MB in serum was examined through ELISA. (C) The level of cTnl in serum was measured through ELISA. (D) The Fe2+ level was tested through the corresponding commercial kit. (E) The levels of MDA and SOD were measured through ELISA.

Article Snippet: Detection of Fe2+ The Fe2+ assay kit (E-BC-K304-S, Elabsciences, China) was used for measuring Fe2+ level as per manufacturer’s protocols.

Techniques: Over Expression, Western Blot, Enzyme-linked Immunosorbent Assay

Journal: Viruses

Article Title: Infectious Spleen and Kidney Necrosis Virus Triggers Ferroptosis in CPB Cells to Enhance Virus Replication.

doi: 10.3390/v17050713

Figure Lengend Snippet: Figure 3. ISKNV infection induces ferroptosis in CPB cells. (A) Transmission electron microscopy of CPB cells treated with DMSO (72 h), erastin (10 µmol/L, 72 h), and ISKNV (100 MOI, 24 h, 48 h, 72 h). Scale bars = 1 µm. (B) Analysis of Fe2+ levels in CPB cells after treatment with ISKNV (100 MOI) using the fluorescent probe FerroOrange by laser scanning confocal microscopy. Scale bars = 20 µm. (C) Quantitative analysis of the mean fluorescence intensity of (B) using Image J. (D) Analysis of intracellular ROS levels using DCFH-DA staining, and laser scanning confocal microscopy of CPB cells treated with ISKNV (100 MOI) for 24–72 h. Scale bars = 10 µm. (E) Quantitative analysis of the mean fluorescence intensity of (D) using Image J. (F–H) Detection of Fe2+, ROS, and MDA levels in cell lysates treated with ISKNV (100 MOI) for 24–72 h by microplate reader. * p < 0.05, ** p < 0.01, and *** p < 0.001, with p > 0.05 considered not significant (ns).

Article Snippet: The Fe2+ content of the cells was detected by laser scanning confocal microscopy and a microplate reader using the fluorescent probe FerroOrange (Elabscience, E-BC-F101, Wuhan, China).

Techniques: Infection, Transmission Assay, Electron Microscopy, Confocal Microscopy, Fluorescence, Staining

Journal: Chemico-biological interactions

Article Title: Gestational exposure to 1-NP induces ferroptosis in placental trophoblasts via CYP1B1/ERK signaling pathway leading to fetal growth restriction.

doi: 10.1016/j.cbi.2023.110812

Figure Lengend Snippet: Fig. 1. Ferroptosis occurs in the placentas of patients with FGR. A: The concentrations of GSH, SOD, MDA and Fe2+ in FGR placental tissues (n = 10); B: Detection of protein expression of GPX4, SLC7A11 and β-actin in the placental tissues of the normal group and FGR group; C: Analysis of Western blotting results in histological graphs; D: Representative images of immunofluorescence staining of placental tissues of patients in the normal and FGR groups. The nuclei were stained with DAPI (blue), and trophoblast cells were labelled with the specific marker CK7. GPX4 and SLC7A11 were stained with specific antibodies (green) (scale bar = 500 μm). (*, p < 0.05; **, p < 0.01; ***, p < 0.001).

Article Snippet: Detection and analysis of GSH, Fe2+, MDA and SOD concentrations A Glutathione Assay Kit (MAK440, MilliporeSigma, USA), Fe2+ Assay Kit (E1042, Applygen, China), MDA Assay Kit (S0131S, Beyotime Institute of biotechnology, China), and Mn-SOD Assay Kit with WST-8 (S0103, Beyotime Institute of Biotechnology, China) were used to analyze the GSH, Fe2+, MDA and SOD concentration in cells, according to the manufacturer’s protocol.

Techniques: Expressing, Western Blot, Immunofluorescence, Staining, Marker

Journal: Chemico-biological interactions

Article Title: Gestational exposure to 1-NP induces ferroptosis in placental trophoblasts via CYP1B1/ERK signaling pathway leading to fetal growth restriction.

doi: 10.1016/j.cbi.2023.110812

Figure Lengend Snippet: Fig. 4. 1-NP induces ferroptosis in HTR8/SVneo and JEG-3 cells. A: The concentrations of GSH, SOD, MDA and Fe2+ under different concentrations of 1-NP; B: The protein expression levels of GPX4, SLC7A11 and β-actin in HTR8/SVneo and JEG-3 cells under different concentrations of 1-NP; C: Analysis of Western blotting results in histological graphs; D: Representative pictures of the microstructure of HTR8/SVneo in the control group and 1-NP-treated group using transmission electron microscopy (left scale bar = 1 μm; right scale bar = 500 nm); E: Representative confocal microscopy image of ROS stained with DCFH-DA in HTR8/SVneo (Scale bar = 50 μm); F: Representative confocal microscopy image of HTR8/SVneo stained with C11-BODIPY(581/591). The green fluorescence intensity exhibited a positive correlation with the level of lipid peroxides. (Scale bar = 50 μm). (*, p < 0.05; **, p < 0.01; ***, p < 0.001).

Article Snippet: Detection and analysis of GSH, Fe2+, MDA and SOD concentrations A Glutathione Assay Kit (MAK440, MilliporeSigma, USA), Fe2+ Assay Kit (E1042, Applygen, China), MDA Assay Kit (S0131S, Beyotime Institute of biotechnology, China), and Mn-SOD Assay Kit with WST-8 (S0103, Beyotime Institute of Biotechnology, China) were used to analyze the GSH, Fe2+, MDA and SOD concentration in cells, according to the manufacturer’s protocol.

Techniques: Expressing, Western Blot, Control, Transmission Assay, Electron Microscopy, Confocal Microscopy, Staining, Fluorescence

Journal: Chemico-biological interactions

Article Title: Gestational exposure to 1-NP induces ferroptosis in placental trophoblasts via CYP1B1/ERK signaling pathway leading to fetal growth restriction.

doi: 10.1016/j.cbi.2023.110812

Figure Lengend Snippet: Fig. 5. Fer-1 reverses 1-NP-induced ferroptosis and restores cell biological function impairment in HTR8/SVneo and JEG-3 cells. A:The concentrations of GSH, SOD, MDA and Fe2+ in HTR8/SVneo and JEG-3; B: The protein expression level of GPX4, SLC7A11 β-actin in HTR8/SVneo and JEG-3; C: Analysis of Western blotting results in histological graphs; D: Cell survival rate were determined by CCK8 assays; E: Images of wound healing assays at 0 h and 24 h in HTR8/SVneo and JEG-3 (Scale bar = 200 μm); F:The migration area of wound healing assays in each group; G: Images of Transwell invasion assay in HTR8/SVneo and JEG-3 (Scale bar = 50 μm); H: The cell numbers of each field in different groups; I: Tube formation assays to detect the ability of angiogenesis in HTR8/SVneo and JEG-3 (Scale bar = 100 μm); J: The tube length of each group. (*, p < 0.05; **, p < 0.01; ***, p < 0.001).

Article Snippet: Detection and analysis of GSH, Fe2+, MDA and SOD concentrations A Glutathione Assay Kit (MAK440, MilliporeSigma, USA), Fe2+ Assay Kit (E1042, Applygen, China), MDA Assay Kit (S0131S, Beyotime Institute of biotechnology, China), and Mn-SOD Assay Kit with WST-8 (S0103, Beyotime Institute of Biotechnology, China) were used to analyze the GSH, Fe2+, MDA and SOD concentration in cells, according to the manufacturer’s protocol.

Techniques: Expressing, Western Blot, Migration, Transwell Invasion Assay

Journal: Chemico-biological interactions

Article Title: Gestational exposure to 1-NP induces ferroptosis in placental trophoblasts via CYP1B1/ERK signaling pathway leading to fetal growth restriction.

doi: 10.1016/j.cbi.2023.110812

Figure Lengend Snippet: Fig. 8. CYP1B1 is involved in 1-NP-induced ferroptosis in HTR8/SVneo and JEG-3 cells. A: The concentrations of GSH, SOD, MDA and Fe2+ in HTR8/SVneo and JEG- 3 cells; B: Detection of the protein expression levels of GPX4, SLC7A11 and β-actin in HTR8/SVneo and JEG-3 cells after CYP1B1 knockdown via western blotting; C: Analysis of western blotting results in histological graphs; D: Detection of the protein expression levels of GPX4, SLC7A11 and β-actin in HTR8/SVneo and JEG-3 cells after CYP1B1 overexpression via western blotting; E: Analysis of western blotting results in histological graphs; F: Representative confocal microscopy image of ROS stained with DCFH-DA in HTR8/SVneo cells (Scale bar = 50 μm). (*, p < 0.05; **, p < 0.01; ***, p < 0.001).

Article Snippet: Detection and analysis of GSH, Fe2+, MDA and SOD concentrations A Glutathione Assay Kit (MAK440, MilliporeSigma, USA), Fe2+ Assay Kit (E1042, Applygen, China), MDA Assay Kit (S0131S, Beyotime Institute of biotechnology, China), and Mn-SOD Assay Kit with WST-8 (S0103, Beyotime Institute of Biotechnology, China) were used to analyze the GSH, Fe2+, MDA and SOD concentration in cells, according to the manufacturer’s protocol.

Techniques: Expressing, Knockdown, Western Blot, Over Expression, Confocal Microscopy, Staining